The acronym NFB may refer to:

• National Federation of the Blind, organization representing blind people in the United States
• National Federation of Builders, trade association representing small and medium building contractors in the United Kingdom
• National Film Board of Canada, Canada's public film producer and distributor
• Negative feedback, process of feeding back to the input a part of a system's output, so as to reverse the direction of change of the output
• Network of Excellence for Functional Biomaterials, research centre based in Galway, Ireland
• Nouvelle Federation Board, non-fifa football representative organisation
• NF-κB, in molecular biology, often written as NF-B
• Neurofeedback, therapy technique that presents the user with realtime feedback on brainwave activity
• Noise from the Basement, debut album by Canadian singer Skye Sweetnam
• North Fork Bank, which was acquired by Capital One Bank in 2008